Transposon-induced inversion in Antirrhinum modifies nivea gene expression to give a novel flower color pattern under the control of cycloidearadialis

Lister, C., Jackson, D., Martin, C. (November 1993) Transposon-induced inversion in Antirrhinum modifies nivea gene expression to give a novel flower color pattern under the control of cycloidearadialis. Plant Cell, 5 (11). pp. 1541-53. ISSN 1040-4651 (Print)1040-4651

URL: http://www.ncbi.nlm.nih.gov/pubmed/8312739
DOI: 10.1105/tpc.5.11.1541

Abstract

The nivea (niv) gene of Antirrhinum majus encodes chalcone synthase, an enzyme involved in synthesis of anthocyanin pigments. The nivrec:98 allele contains a single copy of the transposon Tam3 inserted at the niv locus. A large chromosomal rearrangement derived from this mutant has been shown to be flanked by two copies of Tam3. In this study, we compared sequences involved in this rearrangement with their progenitor sequences and concluded that the rearrangement is an inversion resulting from an aberrant transposition occurring shortly after replication of Tam3 that left both copies of Tam3 active after the rearrangement. Excision of Tam3 from its position adjacent to the niv coding region resulted in a novel distribution of anthocyanin pigment in the flower tube, caused by the interaction of the new sequences with the remnant of the niv promoter. The new sequences upstream of niv serve both to enhance niv transcription and to redirect the pattern of gene expression, placing niv under the control of the gene cycloidearadialis, which determines the morphogenetic polarity of the flower.

Item Type: Paper
Uncontrolled Keywords: Alleles Anthocyanins/*genetics Base Sequence *Chromosome Inversion Cloning, Molecular *DNA Transposable Elements *Gene Expression Regulation Genes, Plant/*physiology Genetic Linkage Molecular Sequence Data Phenotype Pigmentation/genetics Restriction Mapping Sequence Analysis, DNA
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > transposable elements
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: Matt Covey
Date: November 1993
Date Deposited: 12 May 2014 14:56
Last Modified: 12 May 2014 14:56
PMCID: PMC160384
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30043

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving