DNA- and adenovirus-mediated gene transfer into cardiac muscle

Kass-Eisler, A., Leinwand, L. A. (1997) DNA- and adenovirus-mediated gene transfer into cardiac muscle. Methods in Cell Biology, 52. pp. 423-37. ISSN 0091-679X (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9379963
DOI: 10.1016/S0091-679X(08)60390-8

Abstract

This chapter discusses and compares two methods of gene transfer into the rodent heart: direct plasmid DNA injection and injection of recombinant adenovirus. The predominant use of direct injection into the heart has been to analyze promoter elements in vivo using reporter genes driven by the promoter element of interest. The second general use of in vivo gene transfer is to examine the consequences of expression of a given gene on the structure or function of the heart. This could involve expression of a mutant protein or ectopic expression of an isoform of a protein normally found in another setting. For these purposes DNA injection would not be efficient enough for most forms of phenotypic modification. Therefore, focus has shifted on the use of recombinant adenoviruses. Adenoviruses efficiently infect a large number of replicating and nonreplicating cell types. Their ability to infect nonreplicating cells makes them ideal for use in the heart, because adult cardiac myocytes are terminally differentiated.

Item Type: Paper
Uncontrolled Keywords: Adenoviridae Animals Dna Gene Transfer Techniques Genetic Vectors Heart/ physiology Humans
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene transfer
CSHL Authors:
Communities: Watson School
Depositing User: Kathleen Darby
Date: 1997
Date Deposited: 07 May 2014 17:42
Last Modified: 07 May 2014 17:42
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30022

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