Mechanisms of potentiation by calcium-calmodulin kinase II of postsynaptic sensitivity in rat hippocampal CA1 neurons

Shirke, A. M., Malinow, R. (1997) Mechanisms of potentiation by calcium-calmodulin kinase II of postsynaptic sensitivity in rat hippocampal CA1 neurons. Journal of Neurophysiology, 78 (5). pp. 2682-92.

URL: http://www.ncbi.nlm.nih.gov/pubmed/9356418

Abstract

Mechanisms of potentiation by calcium-calmodulin kinase II of postsynaptic sensitivity in rat hippocampal CA1 neurons. J. Neurophysiol. 78: 2682-2692, 1997. Preactivated recombinant alpha-calcium-calmodulin dependent multifunctional protein kinase II (CaMKII*) was perfused internally into CA1 hippocampal slice neurons to test the effect on synaptic transmission and responses to exogenous application of glutamate analogues. After measurement of baseline transmission, internal perfusion of CaMKII* increased synaptic strength in rat hippocampal neurons and diminished the fraction of synaptic failures. After measurement of baseline responses to applied transmitter, CaMKII* perfusion potentiated responses to kainate but not responses to N-methyl--aspartate. Internal perfusion of CaMKII*potentiated the maximal effect of kainate. Potentiation by CaMKII* did not change the time course of responses to kainate, whereas increasing response size by pharmacologically manipulating desensitization or deactivation rate constants significantly altered the time course of responses. Nonstationary fluctuation analysis of responses to kainate showed a decrease in the coefficient of variation after potentiation by CaMKII*. These data support the hypothesis that CaMKII increases postsynaptic responsiveness by increasing the available number of active alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid/kainate channels and suggests that a similar process may occur during the expression of long-term potentiation.

Item Type: Paper
Additional Information: 0022-3077 (Print) In Vitro Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.
Uncontrolled Keywords: Animals Artifacts Ca(2+)-Calmodulin Dependent Protein Kinase/*pharmacology Drug Synergism Electric Stimulation Hippocampus/*physiology Kainic Acid/pharmacology Models, Neurological Perfusion Pyramidal Cells/drug effects/*physiology Rats Rats, Sprague-Dawley Reaction Time Recombinant Proteins/pharmacology Synapses/drug effects/physiology Synaptic Transmission/drug effects/*physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > CaMKII
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > pyramidal neurons

organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission
CSHL Authors:
Communities: CSHL labs > Malinow lab
Depositing User: Kathleen Darby
Date Deposited: 07 May 2014 17:24
Last Modified: 07 May 2014 17:24
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30020

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