A two-dimensional, diagonal sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique to screen for protease substrates in protein mixtures

Nestler, H. P., Doseff, A. (August 1997) A two-dimensional, diagonal sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique to screen for protease substrates in protein mixtures. Analytical Biochemistry, 251 (1). pp. 122-5. ISSN 0003-2697 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9300095
DOI: 10.1006/abio.1997.2265

Abstract

Proteases have emerged during the past few years as key players in the control of cell fate. Many have been identified and cloned, but to understand their function and to elucidate the pathways in which they are involved, the intracellular substrates processed by each protease must be identified. Even the intensive efforts in genome sequencing and cloning that yield new protein sequences every day do not provide a complete solution: the occurrence of a cleavage motif in a protein sequence is not sufficient for proteolytic susceptibility since folding may prevent access of the protease to the substrate site.

Item Type: Paper
Uncontrolled Keywords: Animals Autoradiography Cattle Electrophoresis, Gel, Two-Dimensional/ methods Endopeptidases/ metabolism Evaluation Studies Molecular Weight Proteins/chemistry/ isolation & purification/ metabolism Research Support, Non-U.S. Gov't Sodium Dodecyl Sulfate Substrate Specificity
Subjects: bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Protease
CSHL Authors:
Communities: CSHL labs > Labeznik lab
CSHL labs > Nestler lab
Depositing User: Kathleen Darby
Date: 15 August 1997
Date Deposited: 07 May 2014 19:06
Last Modified: 07 May 2014 19:06
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30009

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