ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication for chromosome condensation

Kimura, K., Hirano, T. (August 1997) ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication for chromosome condensation. Cell, 90 (4). pp. 625-34. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9288743
DOI: 10.1016/S0092-8674(00)80524-3

Abstract

13S condensin is a five-subunit protein complex that plays a central role in mitotic chromosome condensation in Xenopus egg extracts. Two core subunits of this complex, XCAP-C and XCAP-E, belong to an emerging family of putative ATPases, the SMC family. We report here that 13S condensin has a DNA-stimulated ATPase activity and exhibits a high affinity for structured DNAs such as cruciform DNA. 13S condensin is able to introduce positive supercoils into a closed circular DNA in the presence of bacterial or eukaryotic topoisomerase I. The supercoiling reaction is ATP-dependent. We propose that 13S condensin wraps DNA in a right-handed direction by utilizing the energy of ATP hydrolysis. This reaction may represent a key mechanism underlying the compaction of chromatin fibers during mitosis.

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphate/ metabolism Adenosinetriphosphatase/ metabolism Animals Carrier Proteins/ metabolism Cell Cycle Proteins Chromatin/metabolism Chromosomal Proteins, Non-Histone Chromosomes/ metabolism DNA Helicases DNA Topoisomerases, Type I/metabolism DNA, Circular/metabolism DNA, Superhelical/ metabolism DNA-Binding Proteins/ metabolism Hydrolysis Multiprotein Complexes Nuclear Proteins/ metabolism Nucleic Acid Conformation Oocytes/metabolism Research Support, U.S. Gov't, P.H.S. Xenopus Xenopus Proteins
Subjects: bioinformatics > genomics and proteomics > small molecules > ATP
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Hirano lab
Depositing User: Kathleen Darby
Date: 22 August 1997
Date Deposited: 07 May 2014 14:13
Last Modified: 07 May 2014 14:13
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30007

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