Cleary, M. A., Pendergrast, P. S., Herr, W.
(August 1997)
Structural flexibility in transcription complex formation revealed by protein-DNA photocrosslinking.
Proceedings of the National Academy of Sciences, 94 (16).
pp. 8450-5.
ISSN 0027-8424 (Print)
Abstract
The Oct-1 POU domain binds diverse DNA-sequence elements and forms a higher-order regulatory complex with the herpes simplex virus coregulator VP16. The POU domain contains two separate DNA-binding domains joined by a flexible linker. By protein-DNA photocrosslinking we show that the relative positioning of the two POU DNA-binding domains on DNA varies depending on the nature of the DNA target. On a single VP16-responsive element, the POU domain adopts multiple conformations. To determine the structure of the Oct-1 POU domain in a multiprotein complex with VP16, we allowed VP16 to interact with previously crosslinked POU-domain-DNA complexes and found that VP16 can associate with multiple POU-domain conformations. These results reveal the dynamic potential of a DNA-binding domain in directing transcriptional regulatory complex formation.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Binding Sites
DNA/chemistry/genetics/ metabolism
DNA-Binding Proteins/chemistry/genetics/metabolism
Escherichia coli
Herpes Simplex Virus Protein Vmw65/chemistry/metabolism
Homeodomain Proteins/chemistry/genetics/ metabolism
Host Cell Factor C1
Humans
Octamer Transcription Factor-1
Protein Conformation
Research Support, U.S. Gov't, P.H.S.
Transcription Factors/chemistry/genetics/ metabolism
Transcription, Genetic |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > bacteria > escherichia coli
organism description > virus > herpes simplex virus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > homeodomain protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Herr lab
CSHL labs > Hernandez lab |
| Depositing User: |
Kathleen Darby
|
| Date: |
5 August 1997 |
| Date Deposited: |
07 May 2014 17:59 |
| Last Modified: |
11 Sep 2019 16:30 |
| PMCID: |
PMC22952 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/30003 |
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