Structural flexibility in transcription complex formation revealed by protein-DNA photocrosslinking

Cleary, M. A., Pendergrast, P. S., Herr, W. (1997) Structural flexibility in transcription complex formation revealed by protein-DNA photocrosslinking. Proceedings of the National Academy of Sciences, 94 (16). pp. 8450-5. ISSN 0027-8424 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9237997

Abstract

The Oct-1 POU domain binds diverse DNA-sequence elements and forms a higher-order regulatory complex with the herpes simplex virus coregulator VP16. The POU domain contains two separate DNA-binding domains joined by a flexible linker. By protein-DNA photocrosslinking we show that the relative positioning of the two POU DNA-binding domains on DNA varies depending on the nature of the DNA target. On a single VP16-responsive element, the POU domain adopts multiple conformations. To determine the structure of the Oct-1 POU domain in a multiprotein complex with VP16, we allowed VP16 to interact with previously crosslinked POU-domain-DNA complexes and found that VP16 can associate with multiple POU-domain conformations. These results reveal the dynamic potential of a DNA-binding domain in directing transcriptional regulatory complex formation.

Item Type: Paper
Uncontrolled Keywords: Binding Sites DNA/chemistry/genetics/ metabolism DNA-Binding Proteins/chemistry/genetics/metabolism Escherichia coli Herpes Simplex Virus Protein Vmw65/chemistry/metabolism Homeodomain Proteins/chemistry/genetics/ metabolism Host Cell Factor C1 Humans Octamer Transcription Factor-1 Protein Conformation Research Support, U.S. Gov't, P.H.S. Transcription Factors/chemistry/genetics/ metabolism Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > bacteria > escherichia coli
organism description > virus > herpes simplex virus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > homeodomain protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Herr lab
CSHL labs > Hernandez lab
Depositing User: Kathleen Darby
Date Deposited: 07 May 2014 17:59
Last Modified: 07 May 2014 18:01
PMCID: PMC22952
Related URLs:
URI: http://repository.cshl.edu/id/eprint/30003

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