The Cdc2 protein kinase controls Cdc10/Sct1 complex formation

Connolly, T., Caligiuri, M., Beach, D. (June 1997) The Cdc2 protein kinase controls Cdc10/Sct1 complex formation. Molecular Biology of the Cell, 8 (6). pp. 1105-15. ISSN 1059-1524 (Print)

[img]
Preview
PDF
Beach_MolBiolCell1997.pdf

Download (2576Kb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/9201719
DOI: 10.1091/mbc.8.6.1105

Abstract

In the fission yeast Schizosaccharomyces pombe, the execution of Start requires the activity of the Cdc2 protein kinase and the Cdc10/Sct1 transcription complex. The loss of any of these genes leads to G1 arrest and activation of the mating pathway under appropriate conditions. We have undertaken a genetic and biochemical analysis of these genes and their protein products to elucidate the molecular mechanism that governs the regulation of Start. We demonstrate that serine-196 of Cdc10 is phosphorylated in vivo and provide evidence that suggests that phosphorylation of this residue is required for Cdc10 function. Substitution of serine-196 of Cdc10 with alanine (Cdc10 S196A) leads to inactivation of Cdc10. We show that Cdc10 S196A is incapable of associating with Sct1 to form a heteromeric complex, whereas substitution of this serine with aspartic acid (S196D) restores DNA-binding activity by allowing Cdc10 to associate with Sct1. Furthermore, we demonstrate that Cdc2 activity is required for the formation of the heteromeric Sct1/Cdc10 transcription complex and that the Cdc10 S196D mutation alleviates this requirement. We thus provide biochemical evidence to demonstrate one mechanism by which the Cdc2 protein kinase may regulate Start in the fission yeast cell cycle.

Item Type: Paper
Uncontrolled Keywords: CDC2 Protein Kinase/ physiology Cell Cycle Cell Cycle Proteins/ metabolism Consensus Sequence DNA-Binding Proteins/ metabolism Gene Expression Regulation, Fungal Macromolecular Substances Phosphoserine/metabolism Protein Binding Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Schizosaccharomyces/cytology/ genetics Signal Transduction Transcription Factors/ metabolism Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > yeast > Saccharomyces
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organism description > yeast > Saccharomyces > Schizosaccharomyces pombe
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Kathleen Darby
Date: June 1997
Date Deposited: 07 May 2014 19:59
Last Modified: 07 May 2014 19:59
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29996

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving