Purification and characterization of FBI-1, a cellular factor that binds to the human immunodeficiency virus type 1 inducer of short transcripts

Pessler, F., Pendergrast, P. S., Hernandez, N. (July 1997) Purification and characterization of FBI-1, a cellular factor that binds to the human immunodeficiency virus type 1 inducer of short transcripts. Molecular and Cellular Biology, 17 (7). pp. 3786-98. ISSN 0270-7306 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9199312

Abstract

The human immunodeficiency virus (HIV-1) promoter directs the synthesis of two classes of RNA molecules, short transcripts and full-length transcripts. The synthesis of short transcripts depends on a bipartite DNA element, the inducer of short transcripts (IST), located in large part downstream of the HIV-1 start site of transcription. IST does not require any viral product for function and is thought to direct the assembly of transcription complexes that are incapable of efficient elongation. Nothing is known, however, about the biochemical mechanisms that mediate IST function. Here, we report the identification and purification of a factor that binds specifically to the IST. This factor, FBI-1, recognizes a large bipartite binding site that coincides with the bipartite IST element. It is constituted at least in part by an 86-kDa polypeptide that can be specifically cross-linked to IST. FBI-1 also binds to promoter and attenuation regions of a number of cellular and viral transcription units that are regulated by a transcription elongation block. This observation, together with the observation that the binding of FBI-1 to IST mutants correlates with the ability of these mutants to direct IST function, suggests that FBI-1 may be involved in the establishment of abortive transcription complexes.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Binding Sites DNA-Binding Proteins/isolation & purification/metabolism/ physiology Gene Expression Regulation Gene Expression Regulation, Viral HIV-1/ genetics Hela Cells Humans Molecular Sequence Data Molecular Weight Promoter Regions (Genetics) RNA, Viral/genetics Regulatory Sequences, Nucleic Acid Research Support, U.S. Gov't, P.H.S. Transcription Factors/isolation & purification/ physiology Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Kathleen Darby
Date: July 1997
Date Deposited: 07 May 2014 19:40
Last Modified: 07 May 2014 19:40
PMCID: PMC232230
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29993

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