Characterization of a trimeric complex containing Oct-1, SNAPc, and DNA

Ford, E., Hernandez, N. (June 1997) Characterization of a trimeric complex containing Oct-1, SNAPc, and DNA. Journal of Biological Chemistry, 272 (25). pp. 16048-55. ISSN 0021-9258

URL: http://www.ncbi.nlm.nih.gov/pubmed/9188510

Abstract

The human small nuclear (sn) RNA promoters contain a proximal sequence element (PSE), which recruits the basal transcription factor SNAPc, and a distal sequence element characterized by an octamer sequence, which recruits the POU domain transcription factor Oct-1. The Oct-1 POU domain and SNAPc bind cooperatively to probes containing a PSE and an octamer sequence, and this effect contributes to efficient transcription in vitro. In vivo, however, Oct-1 regions outside of the POU domain can activate snRNA gene transcription. Here, we have examined whether the role of these regions is to contribute to cooperative binding with SNAPc. We find that they indeed improve cooperative binding, but most of the effect is nevertheless mediated by just the POU domain. This suggests that Oct-1 activates transcription of snRNA genes in at least two steps, recruitment of SNAPc mediated primarily by the POU domain, and a later step mediated by regions outside of the POU domain. We also show that a PSE-binding complex observed in nuclear extracts consists of Oct-1 and SNAPc. Although Oct-1 cannot bind effectively to the PSE probe on its own, in the complex it contacts DNA. Thus, in a nuclear extract, SNAPc can recruit Oct-1 to a probe to which Oct-1 cannot bind on its own.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Binding Sites DNA/ metabolism DNA-Binding Proteins/ metabolism Electrophoresis, Polyacrylamide Gel Host Cell Factor C1 Humans Macromolecular Substances Molecular Sequence Data Octamer Transcription Factor-1 Octamer Transcription Factor-2 Proteins Research Support, U.S. Gov't, P.H.S. Transcription Factors/ metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Kathleen Darby
Date: 20 June 1997
Date Deposited: 07 May 2014 19:31
Last Modified: 07 May 2014 19:31
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29992

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