Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein

Hirano, T., Kobayashi, R., Hirano, M. (1997) Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein. Cell, 89 (4). pp. 511-21. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9160743
DOI: 10.1016/S0092-8674(00)80233-0

Abstract

We report here purification and characterization of chromosome condensation protein complexes (termed condensins) containing XCAP-C and XCAP-E, two Xenopus members of the SMC family. Sucrose density gradient centrifugation reveals two major forms of condensins. The 8S form is a heterodimer of XCAP-C and XCAP-E, whereas the 13S form contains three additional subunits. One of them is identified as a homolog of the Drosophila Barren protein whose mutation shows a defect in chromosome segregation. Chromosomal targeting of condensins is mitosis-specific and is independent of topoisomerase IIalpha. 13S condensin is required for condensation, as demonstrated by immunodepletion and rescue experiments. Our results suggest that the condensin complexes represent the most abundant structural components of mitotic chromosomes and play a central role in driving chromosome condensation.

Item Type: Paper
Uncontrolled Keywords: Adenosinetriphosphatase/chemistry/genetics/ isolation & purification Amino Acid Sequence Animals Antigens, Neoplasm Carrier Proteins/chemistry/genetics/ isolation & purification Cell Cycle Cell Cycle Proteins/chemistry/genetics/ isolation & purification Centrifugation, Density Gradient Chromosomal Proteins, Non-Histone/chemistry/genetics/ isolation & purification Chromosomes/chemistry DNA Topoisomerases, Type II/metabolism DNA Topoisomerases, Type II, Eukaryotic DNA-Binding Proteins/chemistry/genetics/ isolation & purification Dimerization Drosophila Drosophila Proteins Female Isoenzymes/metabolism Macromolecular Substances Mitosis Molecular Sequence Data Multiprotein Complexes Nuclear Proteins/chemistry/genetics/ isolation & purification Ovum/chemistry Phosphorylation Protein Conformation Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Sequence Homology, Amino Acid Species Specificity Xenopus Xenopus Proteins
Subjects: organism description > animal > insect > Drosophila
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > condensin complex
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Hirano lab
CSHL labs > Kobayashi lab
Depositing User: Kathleen Darby
Date Deposited: 08 May 2014 15:45
Last Modified: 08 May 2014 15:45
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29989

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