Role for the amino-terminal region of human TBP in U6 snRNA transcription

Mittal, V., Hernandez, N. (February 1997) Role for the amino-terminal region of human TBP in U6 snRNA transcription. Science, 275 (5303). pp. 1136-40. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9027316
DOI: 10.1126/science.275.5303.1136

Abstract

Basal transcription from the human RNA polymerase III U6 promoter depends on a TATA box that recruits the TATA box-binding protein (TBP) and a proximal sequence element that recruits the small nuclear RNA (snRNA)-activating protein complex (SNAPc). TBP consists of a conserved carboxyl-terminal domain that performs all known functions of the protein and a nonconserved amino-terminal region of unknown function. Here, the amino-terminal region is shown to down-regulate binding of TBP to the U6 TATA box, mediate cooperative binding with SNAPc to the U6 promoter, and enhance U6 transcription.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence DNA Footprinting DNA-Binding Proteins/chemistry/genetics/ metabolism Down-Regulation Humans Molecular Sequence Data Promoter Regions (Genetics) Proteins/metabolism RNA, Small Nuclear/ genetics RNA-Binding Proteins/metabolism Recombinant Fusion Proteins/chemistry/metabolism Research Support, U.S. Gov't, P.H.S. TATA Box TATA-Box Binding Protein Transcription Factors/chemistry/genetics/ metabolism Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > RNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Hernandez lab
CSHL labs > Mittal lab
Depositing User: Kathleen Darby
Date: 21 February 1997
Date Deposited: 08 May 2014 18:36
Last Modified: 08 May 2014 18:36
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29971

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