Expression of CDC2Zm and KNOTTED1 during in-vitro axillary shoot meristem proliferation and adventitious shoot meristem formation in maize (Zea mays L.) and barley (Hordeum vulgare L.)

Zhang, S., Williams-Carrier, R., Jackson, D., Lemaux, P. G. (1998) Expression of CDC2Zm and KNOTTED1 during in-vitro axillary shoot meristem proliferation and adventitious shoot meristem formation in maize (Zea mays L.) and barley (Hordeum vulgare L.). Planta, 204 (4). pp. 542-549. ISSN 00320935 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9684373
DOI: 10.1007/s004250050289

Abstract

Expression of CDC2Zm and KNOTTED1 (KN1) in maize (Zea mays L.) and their cross-reacting proteins in barley (Hordeum vulgate L.) was studied using immunolocalization during in-vitro axillary shoot meristem proliferation and adventitious shoot meristem formation. Expression of CDC2Zm, a protein involved in cell division, roughly correlated with in-vitro cell proliferation and in the meristematic domes CDC2Zm expression was triggered during in-vitro proliferation. Analysis of the expression of KN1, a protein necessary for maintenance of the shoot meristem, showed that KN1 or KN1-homologue(s) expression was retained in meristematic cells during in-vitro proliferation of axillary shoot meristems. Multiple adventitious shoot meristems appeared to form directly from the KN1- or KN1 homologue(s)-expressing meristematic cells in the in-vitro proliferating meristematic domes. However, unlike Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum) leaves ectopically expressing KN1 (G. Chuck et al., 1996 Plant Cell 8: 1277-1289; N. Sinha et al., 1993 Genes Dev. 7: 787-797), transgenic maize leaves over-expressing KN1 were unable to initiate adventitious shoot meristems on their surfaces either in planta or in vitro. Therefore, expression of KN1 is not the sole triggering factor responsible for inducing adventitious shoot meristem formation from in-vitro proliferating axillary shoot meristems in maize. Our results show that genes critical to cell division and plant development have utility in defining in-vitro plant morphogenesis at the molecular level and, in combination with transformation technologies, will be powerful tools in identifying the fundamental molecular and-or genetic triggering factor(s) responsible for reprogramming of plant cells during plant morphogenesis in-vitro.

Item Type: Paper
Uncontrolled Keywords: Gene expression (CDC2Zm, KN1) Hordeum (shoot meristem) Organogenesis (in vitro) Shoot meristem Zea (shoot meristem) axillary shoot barley CDC2Zm gene gene expression in vitro plant morphogenesis KNOTTED1 gene maize plant growth plant morphogenesis tobacco transgenic plant Antibodies CDC2 Protein Kinase Cell Division Homeodomain Proteins Hordeum Immunoenzyme Techniques Meristem Nuclear Proteins Plant Leaves Plant Proteins Plant Shoots Plants, Genetically Modified Zea mays Arabidopsis Arabidopsis thaliana Embryophyta Hordeum vulgare subsp. vulgare Hordeum vulgate Nicotiana tabacum Tabacum Zea
Subjects: organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > antibodies
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > homeodomain protein
organs, tissues, organelles, cell types and functions > tissues types and functions > meristem
organism description > plant
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: Kathleen Darby
Date Deposited: 05 May 2014 15:30
Last Modified: 02 Sep 2014 18:46
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29946

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