Stabilization of dendritic arbor structure in vivo by CaMKII

Wu, G. Y., Cline, H. T. (1998) Stabilization of dendritic arbor structure in vivo by CaMKII. Science, 279 (5348). pp. 222-6. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9422694
DOI: 10.1126/science.279.5348.222

Abstract

Calcium-calmodulin-dependent protein kinase II (CaMKII) promotes the maturation of retinotectal glutamatergic synapses in Xenopus. Whether CaMKII activity also controls morphological maturation of optic tectal neurons was tested using in vivo time-lapse imaging of single neurons over periods of up to 5 days. Dendritic arbor elaboration slows with maturation, in correlation with the onset of CaMKII expression. Elevating CaMKII activity in young neurons by viral expression of constitutively active CaMKII slowed dendritic growth to a rate comparable to that of mature neurons. CaMKII overexpression stabilized dendritic structure in more mature neurons, whereas CaMKII inhibition increased their dendritic growth. Thus, endogenous CaMKII activity limits dendritic growth and stabilizes dendrites, and it may act as an activity-dependent mediator of neuronal maturation.

Item Type: Paper
Uncontrolled Keywords: Animals Axons/physiology/ultrastructure Benzylamines/pharmacology Ca(2+)-Calmodulin Dependent Protein Kinase/ metabolism Carbocyanines Dendrites/physiology/ ultrastructure Fluorescent Dyes Microscopy, Confocal Neurons/cytology/ enzymology/physiology/ ultrastructure Receptors, AMPA/metabolism Receptors, N-Methyl-D-Aspartate/metabolism Recombinant Proteins/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Sulfonamides/pharmacology Superior Colliculus/ cytology/enzymology Synapses/metabolism Synaptic Transmission Time Factors Vaccinia virus/genetics Xenopus laevis
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > CaMKII
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors

Investigative techniques and equipment > microscopy
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Cline lab
Depositing User: Kathleen Darby
Date Deposited: 01 May 2014 13:53
Last Modified: 01 May 2014 13:53
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29941

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