Two-hybrid analysis of Ras-Raf interactions

Van Aelst, L. (1998) Two-hybrid analysis of Ras-Raf interactions. Methods in Molecular Biology, 84. pp. 201-22. ISSN 1064-3745 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9666451
DOI: 10.1385/0-89603-488-7:201

Abstract

The yeast two-hybrid system (1) is a genetic method that enables the experimentor to determine whether two proteins can form complexes within yeast cells. The method comprises expressing the proteins of interest as “hybrid” proteins, one fused to a DNA-binding domain and the other protein fused to a transcription-activating domain. If the fusion proteins interact, a reporter gene IS transcribed

Item Type: Paper
Uncontrolled Keywords: Genetic Techniques Oncogene Proteins v-raf Protein Binding Retroviridae Proteins, Oncogenic/isolation & purification/ metabolism Saccharomyces cerevisiae/genetics/metabolism Transformation, Genetic ras Proteins/isolation & purification/ metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Van Aelst lab
Depositing User: Kathleen Darby
Date: 1998
Date Deposited: 01 May 2014 13:16
Last Modified: 01 May 2014 13:16
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29938

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