Identification, sequence analysis and expression studies of novel anther-specific genes of Arabidopsis thaliana

Rubinelli, P., Hu, Y., Ma, H. (July 1998) Identification, sequence analysis and expression studies of novel anther-specific genes of Arabidopsis thaliana. Plant Molecular Biology, 37 (4). pp. 607-19. ISSN 0167-4412 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9687065
DOI: 10.1023/A:1005964431302

Abstract

Relatively little is known about pollen development at the molecular level. For the purpose of gaining understanding of the molecular control of pollen development, a number of Arabidopsis cDNA fragments were isolated using subtractive hybridizations. DNA and RNA hybridizations and sequence analyses indicate that we have isolated cDNAs representing 13 genes. Sequences for 8 of these genes are novel, while those for the remaining 5 genes have substantial similarity to genes previously reported as anther- or pollen-specific. RNA in situ hybridizations with 5 genes revealed that four of them are tapetum-specific with differing temporal expression patterns during pollen development and one is pollen-specific within the flower. Sequence analysis of full-length cDNAs showed that one of the novel genes, ATA7, encodes a protein related to lipid transfer proteins. Another gene, ATA20, encodes a protein with novel repeat sequences and a glycine-rich domain that shares a predicted structure with a known cell wall protein. The full-length ATA27 cDNA encodes a protein similar to the BGL4 beta-glucosidase from Brassica napus. The ATA27 protein is predicted to have an ER retention signal and an acidic isoelectric point, suggesting that it may be localized to the ER lumen. This may be a means of compartmentalization from its substrate(s). Our studies demonstrate that subtractive hybridizations can be used to identify previously unknown genes, which should be valuable tools for further study of pollen and anther development and function.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Arabidopsis/ genetics/growth & development/metabolism Base Sequence Carrier Proteins/genetics Comparative Study DNA Primers/genetics DNA, Complementary/genetics/isolation & purification DNA, Plant/genetics/isolation & purification Genes, Plant In Situ Hybridization Molecular Sequence Data Plant Proteins/genetics Pollen/ genetics/growth & development Polymerase Chain Reaction RNA, Messenger/genetics/metabolism RNA, Plant/genetics/metabolism Repetitive Sequences, Nucleic Acid Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Sequence Homology, Amino Acid beta-Glucosidase/genetics
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
organism description > plant
CSHL Authors:
Communities: CSHL labs
Depositing User: Kathleen Darby
Date: July 1998
Date Deposited: 01 May 2014 15:08
Last Modified: 07 May 2014 13:07
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29926

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