The HIV-1 inducer of short transcripts activates the synthesis of 5,6-dichloro-1-beta-D-benzimidazole-resistant short transcripts in vitro

Pessler, F., Hernandez, N. (February 1998) The HIV-1 inducer of short transcripts activates the synthesis of 5,6-dichloro-1-beta-D-benzimidazole-resistant short transcripts in vitro. Journal of Biological Chemistry, 273 (9). pp. 5375-84. ISSN 0021-9258 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9478998
DOI: 10.1074/jbc.273.9.5375

Abstract

The HIV-1 inducer of short transcripts (IST) is an unusual promoter element that activates the synthesis of short transcripts from the HIV-1 promoter as well as from heterologous promoters. While the DNA sequences constituting IST have been characterized in some detail, little is known about the biochemical mechanisms underlying IST activity. Here, we describe a cell-free transcription assay that faithfully reproduces the synthesis of IST-dependent HIV-1 short transcripts. As in vivo, formation of these short transcripts requires a functional IST element and is repressed in the presence of the viral trans-activator Tat. Short transcript and full-length transcript synthesis respond differently to variations in several reaction parameters, suggesting that the short and full-length transcripts are synthesized by transcription complexes with distinct biochemical properties. In particular, short transcript synthesis is resistant to the action of 5,6-dichloro-1-beta-D-benzimidazole, an inhibitor of transcript elongation. Formation of transcription complexes directed by the IST element may, therefore, not require the activity of a factor inhibited by 5, 6-dichloro-1-beta-D-benzimidazole, such as the TFIIH-associated or pTEFb kinases.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Benzimidazoles/pharmacology Cell-Free System DNA, Viral/genetics Drug Resistance, Microbial/genetics HIV-1/ genetics Molecular Sequence Data Mutation Promoter Regions (Genetics) RNA Polymerase II/ metabolism RNA, Viral/ biosynthesis Transcription, Genetic/drug effects
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
diseases & disorders > viral diseases
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Kathleen Darby
Date: 27 February 1998
Date Deposited: 01 May 2014 14:27
Last Modified: 01 May 2014 14:27
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29921

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