Use-dependent AMPA receptor block in mice lacking GluR2 suggests postsynaptic site for LTP expression

Mainen, Z. F., Jia, Z., Roder, J., Malinow, R. (November 1998) Use-dependent AMPA receptor block in mice lacking GluR2 suggests postsynaptic site for LTP expression. Nature Neuroscience, 1 (7). pp. 579-86. ISSN 1097-6256

URL: http://www.ncbi.nlm.nih.gov/pubmed/10196565
DOI: 10.1038/2812

Abstract

The mechanisms responsible for enhanced transmission during long-term potentiation (LTP) at CA1 hippocampal synapses remain elusive. Several popular models for LTP expression propose an increase in 'use' of existing synaptic elements, such as increased probability of transmitter release or increased opening of postsynaptic receptors. To test these models directly, we studied a GluR2 knockout mouse in which AMPA receptor transmission is rendered sensitive to a use-dependent block by polyamine compounds. This method can detect increases during manipulations affecting transmitter release or AMPA receptor channel open time and probability, but shows no such changes during LTP. Our results indicate that the recruitment of new AMPA receptors and/or an increase in the conductance of these receptors is responsible for the expression of CA1 LTP.

Item Type: Paper
Uncontrolled Keywords: Action Potentials/physiology Animals Excitatory Postsynaptic Potentials/physiology In Vitro Long-Term Potentiation/ physiology Mice Mice, Knockout/ genetics/ metabolism/physiology Polyamines/pharmacology Receptors, AMPA/ antagonists & inhibitors/ genetics/physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Synapses/ physiology Synaptic Transmission/physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > Long term potentiation
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Communities: CSHL labs > Mainen lab
CSHL labs > Malinow lab
Depositing User: Kathleen Darby
Date: November 1998
Date Deposited: 01 May 2014 16:58
Last Modified: 01 May 2014 16:58
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29912

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving