Candidate adaptor protein CED-6 promotes the engulfment of apoptotic cells in C. elegans

Liu, Q. A., Hengartner, M. O. (June 1998) Candidate adaptor protein CED-6 promotes the engulfment of apoptotic cells in C. elegans. Cell, 93 (6). pp. 961-72. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9635426
DOI: 10.1016/S0092-8674(00)81202-7

Abstract

The rapid engulfment (phagocytosis) of cells undergoing programmed cell death (apoptosis) is a fundamental biological process that is not well understood. Here we report the cloning and functional characterization of ced-6, a gene specifically required for the engulfment of apoptotic cells in the nematode C. elegans. The CED-6 protein contains a phosphotyrosine binding domain at its N terminus and a proline/serine-rich region in its C-terminal half. Genetic mosaic analysis demonstrates that ced-6 acts within engulfing cells. We also show that ced-6 can promote the engulfment of cells at both early and late stages of apoptosis. Our data suggest that CED-6 is an adaptor molecule acting in a signal transduction pathway that specifically mediates the recognition and engulfment of apoptotic cells.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Animals, Genetically Modified Apoptosis/ genetics Base Sequence Caenorhabditis elegans/ cytology/genetics Caenorhabditis elegans Proteins Cloning, Molecular Genes, Helminth/genetics/physiology Molecular Sequence Data Mosaicism Mutation Phagocytosis/ genetics Phosphoproteins/genetics/ physiology Phosphotyrosine/metabolism RNA, Helminth/analysis RNA, Messenger/analysis Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Sequence Homology, Amino Acid Signal Transduction src Homology Domains
Subjects: organism description > animal > C elegans
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > phagocytosis
organs, tissues, organelles, cell types and functions > tissues types and functions > signal transduction
CSHL Authors:
Communities: CSHL labs > Hengartner lab
Depositing User: Kathleen Darby
Date: 12 June 1998
Date Deposited: 01 May 2014 16:33
Last Modified: 01 May 2014 16:33
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29908

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