Arabidopsis Rho-related GTPases: differential gene expression in pollen and polar localization in fission yeast

Li, H., Wu, G., Ware, D., Davis, K. R., Yang, Z. (1998) Arabidopsis Rho-related GTPases: differential gene expression in pollen and polar localization in fission yeast. Plant Physiology, 118 (2). pp. 407-17. ISSN 0032-0889 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/9765526
DOI: 10.​1104/​pp.​118.​2.​407

Abstract

The Rho small GTP-binding proteins are versatile, conserved molecular switches in eukaryotic signal transduction. Plants contain a unique subfamily of Rho-GTPases called Rop (Rho-related GTPases from plants). Our previous studies involving injection of antibodies indicated that the pea Rop GTPase Rop1Ps is critical for pollen tube growth. In this study we show that overexpression of an apparent Arabidopsis ortholog of Rop1Ps, Rop1At, induces isotropic cell growth in fission yeast (Schizosaccharomyces pombe) and that green fluorescence protein-tagged Rop1At displays polar localization to the site of growth in yeast. We found that Rop1At and two other Arabidopsis Rops, Rop3At and Rop5At, are all expressed in mature pollen. All three pollen Rops fall into the same subgroup as Rop1Ps and diverge from those Rops that are not expressed in mature pollen, suggesting a coupling of the structural conservation of Rop GTPases to their gene expression in pollen. However, pollen-specific transcript accumulation for Rop1At is much higher than that for Rop3At and Rop5At. Furthermore, Rop1At is specifically expressed in anthers, whereas Rop3At and Rop5At are also expressed in vegetative tissues. In transgenic plants containing the Rop1At promoter:GUS fusion gene, GUS is specifically expressed in mature pollen and pollen tubes. We propose that Rop1At may play a predominant role in the regulation of polarized cell growth in pollen, whereas its close relatives Rop3At and Rop5At may be functionally redundant to Rop1At in pollen.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Arabidopsis/ enzymology/genetics Base Sequence Cell Division/genetics Cloning, Molecular GTP Phosphohydrolases/ genetics/metabolism GTP-Binding Proteins/ metabolism Gene Expression Regulation, Fungal Gene Expression Regulation, Plant Molecular Sequence Data Pollen/ enzymology Schizosaccharomyces/ genetics Sequence Homology, Amino Acid
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Rho
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > GTP binding protein
organism description > yeast > Saccharomyces > Schizosaccharomyces pombe
CSHL Authors:
Communities: CSHL labs > Ware lab
Depositing User: Kathleen Darby
Date: October 1998
Date Deposited: 01 May 2014 15:47
Last Modified: 01 May 2014 15:47
PMCID: PMC34816
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29906

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