SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III

Henry, R. W., Mittal, V., Ma, B., Kobayashi, R., Hernandez, N. (September 1998) SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III. Genes and Development, 12 (17). pp. 2664-72. ISSN 08909369 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/9732265
DOI: 10.1101/gad.12.17.2664

Abstract

The basal transcription factor SNAPc binds to the PSE, a core element in the RNA polymerase II and III human snRNA promoters. SNAPc contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAPc. Here we reconstitute SNAPc from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAPc nucleates the assembly of two classes of initiation complexes.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Hela Cells Humans Macromolecular Substances Models, Molecular Molecular Sequence Data Promoter Regions (Genetics) RNA Polymerase II/ metabolism RNA Polymerase III/ metabolism RNA, Small Nuclear/ genetics/metabolism Recombinant Proteins/biosynthesis/chemistry/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Transcription Factors/biosynthesis/chemistry/genetics/ metabolism Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > SNAP protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Hernandez lab
CSHL labs > Kobayashi lab
CSHL labs > Mittal lab
CSHL labs > Wigler lab
Depositing User: Kathleen Darby
Date: 1 September 1998
Date Deposited: 01 May 2014 18:04
Last Modified: 07 May 2014 13:14
PMCID: PMC317148
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29894

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