The Oct-1 POU domain activates snRNA gene transcription by contacting a region in the SNAPc largest subunit that bears sequence similarities to the Oct-1 coactivator OBF-1

Ford, E., Strubin, M., Hernandez, N. (1998) The Oct-1 POU domain activates snRNA gene transcription by contacting a region in the SNAPc largest subunit that bears sequence similarities to the Oct-1 coactivator OBF-1. Genes and Development, 12 (22). pp. 3528-40. ISSN 08909369 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/9832505
DOI: 10.1101/gad.12.22.3528

Abstract

The RNA polymerases II and III snRNA gene promoters contain an octamer sequence as part of the enhancer and a proximal sequence element (PSE) as part of the core promoter. The octamer and the PSE bind the POU domain activator Oct-1 and the basal transcription factor SNAPc, respectively. Oct-1, but not Oct-1 with a single E7R mutation within the POU domain, binds cooperatively with SNAPc and, in effect, recruits SNAPc to the PSE. Here, we show that SNAPc recruitment is mediated by an interaction between the Oct-1 POU domain and a small region of the largest subunit of SNAPc, SNAP190. This SNAP190 region is strikingly similar to a region in the B-cell-specific Oct-1 coactivator, OBF-1, that is required for interaction with octamer-bound Oct-1 POU domain. The Oct-1 POU domain-SNAP190 interaction is a direct protein-protein contact as determined by the isolation of a switched specificity SNAP190 mutant that interacts with Oct-1 POU E7R but not with wild-type Oct-1 POU. We also show that this direct protein-protein contact results in activation of transcription. Thus, we have identified an activation target of a human activator, Oct-1, within its cognate basal transcription complex.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Binding Sites/genetics DNA Probes/genetics/metabolism DNA-Binding Proteins/ genetics/metabolism Homeodomain Proteins/genetics Host Cell Factor C1 Humans Molecular Sequence Data Mutation/genetics Octamer Transcription Factor-1 Promoter Regions (Genetics)/genetics Protein Binding/genetics RNA Polymerase II/genetics RNA Polymerase III/genetics RNA, Small Nuclear/ genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Trans-Activation (Genetics)/ genetics Trans-Activators/ genetics/metabolism Transcription Factors/ genetics/metabolism
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Kathleen Darby
Date Deposited: 05 May 2014 15:01
Last Modified: 07 May 2014 13:26
PMCID: PMC317248
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29879

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