Postsynaptic calcium/calmodulin-dependent protein kinase II is required to limit elaboration of presynaptic and postsynaptic neuronal arbors

Zou, D. J., Cline, H. T. (1999) Postsynaptic calcium/calmodulin-dependent protein kinase II is required to limit elaboration of presynaptic and postsynaptic neuronal arbors. Journal of Neuroscience, 19 (20). pp. 8909-18. ISSN 1529-2401 (Electronic)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/10516310

Abstract

Neuronal dendritic and axonal arbors grow to a characteristic size and then stabilize their structures. Activity-dependent stop-growing signals may limit neuronal process elaboration. We tested whether endogenous calcium/calmodulin-dependent protein kinase II (CaMKII) activity in postsynaptic optic tectal cells is required to restrict the elaboration of neuronal processes in the Xenopus tadpole retinotectal projection. Optic tectal cells were infected with vaccinia viruses that express CaMKII-specific inhibitory peptides. In vivo time-lapse imaging revealed that expression of CaMKII inhibitors blocked the growth restriction that normally occurs during maturation of tectal cell dendritic arbors. Postsynaptic CaMKII inhibition also increased the growth of presynaptic retinotectal axon arbors. The results indicate that endogenous postsynaptic CaMKII activity is required to limit the growth of presynaptic and postsynaptic arbor structures in vivo.

Item Type: Paper
Uncontrolled Keywords: Animals Axons/physiology Ca(2+)-Calmodulin Dependent Protein Kinase/antagonists & inhibitors/metabolism/ physiology Cell Division/physiology Dendrites/ physiology Enzyme Inhibitors/metabolism Larva Presynaptic Terminals/ physiology Research Support, U.S. Gov't, P.H.S. Retina/physiology Superior Colliculus/cytology/enzymology/virology Synapses/enzymology/ physiology Vaccinia virus/metabolism Xenopus/growth & development
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > CaMKII
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division
organs, tissues, organelles, cell types and functions > cell types and functions
organs, tissues, organelles, cell types and functions > tissues types and functions > retina
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Cline lab
Depositing User: Kathleen Darby
Date Deposited: 23 Apr 2014 19:14
Last Modified: 30 Apr 2014 17:24
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29847

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