Developmental expression of an amn(+) transgene rescues the mutant memory defect of amnesiac adults

DeZazzo, J., Xia, S., Christensen, J., Velinzon, K., Tully, T. (1999) Developmental expression of an amn(+) transgene rescues the mutant memory defect of amnesiac adults. Journal of Neuroscience, 19 (20). pp. 8740-6. ISSN 1529-2401 (Electronic)

[img]
Preview
PDF
Tully_JNeurosci1999.pdf

Download (226Kb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/10516293

Abstract

The Drosophila memory gene amnesiac (amn) has been proposed to encode a neuropeptide protein, which includes regions homologous to vertebrate pituitary adenylyl cyclase-activating peptide (PACAP; Feany and Quinn, 1995). Definitive experiments to link this gene to memory formation, however, have not yet been accomplished (Kandel and Abel, 1995). The experiments described here demonstrate that the putative amn transcript is involved in adult memory formation. With the use of a UAS-amn(+) transgene, we show complete rescue of memory defects in amn(28A), a mutant allele caused by the insertion of a GAL4 enhancer trap transposon (Moore et al., 1998). Study of the amn(28A) reporter reveals widespread expression in the adult brain but also enriched expression in the embryonic and larval nervous systems. To begin addressing the temporal requirement of amn in memory, we asked whether the memory defects could be rescued by restricting transgenic expression to the adult stage. A heat-shock regimen shown previously to rescue fully the amn ethanol sensitivity defect (Moore et al., 1998) failed to rescue the memory defect. These results, coupled with previous genetic and anatomical studies, suggest that adult memory formation and ethanol sensitivity have different temporal and spatial requirements for amn.

Item Type: Paper
Uncontrolled Keywords: Aging/ physiology Alleles Animals Drosophila/ genetics/ growth & development Gene Expression/physiology Gene Expression Regulation, Developmental Heat Memory Disorders/ genetics Mutation/ genetics Olfactory Pathways/physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Shock/genetics/physiopathology Transgenes/ physiology
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > animal behavior > memory
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
organism description > animal behavior > olfactory
CSHL Authors:
Communities: CSHL labs > Herr lab
CSHL labs > Tully lab
Depositing User: Kathleen Darby
Date Deposited: 23 Apr 2014 19:05
Last Modified: 23 Apr 2014 19:05
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29846

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving