13S condensin actively reconfigures DNA by introducing global positive writhe: implications for chromosome condensation

Kimura, K., Rybenkov, V. V., Crisona, N. J., Hirano, T., Cozzarelli, N. R. (July 1999) 13S condensin actively reconfigures DNA by introducing global positive writhe: implications for chromosome condensation. Cell, 98 (2). pp. 239-48. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10428035
DOI: 10.1016/S0092-8674(00)81018-1

Abstract

Xenopus 13S condensin converts interphase chromatin into mitotic-like chromosomes, and, in the presence of ATP and a type I topoisomerase, introduces (+) supercoils into DNA. The specific production of (+) trefoil knots in the presence of condensin and a type II topoisomerase shows that condensin reconfigures DNA by introducing an ordered, global, (+) writhe. Knotting required ATP hydrolysis and cell cycle-specific phosphorylation of condensin. Condensin bound preferentially to (+) supercoiled DNA in the presence of ATP but not in its absence. Our results suggest a mechanism for the compaction of chromatin by condensin during mitosis.

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphate/metabolism Adenosinetriphosphatase/ metabolism Animals Cell Cycle/physiology Chromosomes/chemistry/ enzymology DNA Topoisomerases, Type I/metabolism DNA Topoisomerases, Type II/metabolism DNA, Superhelical/ chemistry/metabolism/ultrastructure DNA-Binding Proteins/ metabolism Hydrolysis Kinetics Microscopy, Electron Mitosis/physiology Multiprotein Complexes Nucleic Acid Conformation Oocytes/physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Xenopus
Subjects: bioinformatics > genomics and proteomics > small molecules > ATP
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
Investigative techniques and equipment > microscopy > electron microscopy
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Hirano lab
Depositing User: Kathleen Darby
Date: 23 July 1999
Date Deposited: 28 Apr 2014 14:25
Last Modified: 28 Apr 2014 14:25
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29832

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