Crystal structure of the conserved core of the herpes simplex virus transcriptional regulatory protein VP16

Liu, Y., Gong, W., Huang, C. C., Herr, W., Cheng, X. (1999) Crystal structure of the conserved core of the herpes simplex virus transcriptional regulatory protein VP16. Genes and Development, 13 (13). pp. 1692-703. ISSN 0890-9369 (Print)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/10398682

Abstract

On infection, the herpes simplex virus (HSV) virion protein VP16 (Vmw65; alphaTIF) forms a transcriptional regulatory complex-the VP16-induced complex-with two cellular proteins, HCF and Oct-1, on VP16-responsive cis-regulatory elements in HSV immediate-early promoters called TAATGARAT. Comparison of different HSV VP16 sequences reveals a conserved core region that is sufficient for VP16-induced complex formation. The crystal structure of the VP16 core has been determined at 2.1 A resolution. The results reveal a novel, seat-like protein structure. Together with the activity of mutant VP16 proteins, the structure of free VP16 suggests that it contains (1) a disordered carboxy-terminal region that associates with HCF, Oct-1, and DNA in the VP16-induced complex, and (2) a structured region involved in virion assembly and possessing a novel DNA-binding surface that differentiates among TAATGARAT VP16-response elements.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Comparative Study Crystallization Crystallography, X-Ray DNA/metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation, Viral Herpes Simplex Virus Protein Vmw65/ chemistry/genetics/metabolism Herpesviridae/chemistry Host Cell Factor C1 Macromolecular Substances Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Octamer Transcription Factor-1 Protein Conformation Recombinant Fusion Proteins/genetics Regulatory Sequences, Nucleic Acid Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Sequence Alignment Sequence Homology, Amino Acid Simplexvirus/ chemistry/physiology Trans-Activation (Genetics) Transcription Factors/metabolism
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > virus > herpes simplex virus
Investigative techniques and equipment > x ray crystallography
CSHL Authors:
Communities: CSHL labs > Herr lab
CSHL labs > Xu lab
Depositing User: Kathleen Darby
Date Deposited: 28 Apr 2014 13:34
Last Modified: 30 Apr 2014 17:33
PMCID: PMC316849
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29828

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