A human DNA editing enzyme homologous to the Escherichia coli DnaQ/MutD protein

Hoss, M., Robins, P., Naven, T. J., Pappin, D. J., Sgouros, J., Lindahl, T. (July 1999) A human DNA editing enzyme homologous to the Escherichia coli DnaQ/MutD protein. EMBO Journal, 18 (13). pp. 3868-75. ISSN 0261-4189 (Print)0261-4189 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10393201
DOI: 10.1093/emboj/18.13.3868

Abstract

Mammalian DNA polymerases alpha and beta lack 3' exonuclease activity and are unable to edit errors after DNA synthesis. However, editing exonucleases can be functions of separate polypeptides. We isolated a widely distributed DNA-specific 3' exonuclease from rabbit liver nuclei, sequenced tryptic peptides by mass spectrometry, and identified the corresponding human open reading frame. The protein expressed from the cloned human sequence exhibits 3' exonuclease activity. The human clone shares sequence homology with the editing function of the Escherichia coli DNA polymerase III holoenzyme, i.e., the DnaQ/MutD protein, and weakly with the editing 3' exonuclease domain of eukaryotic DNA polymerase epsilon. The gene maps to human chromosome 3p21.2-21.3. In a reconstituted human DNA repair system containing DNA polymerase beta and DNA ligase III-XRCC1, accurate rejoining of a 3' mismatched base residue at a single-strand break is dependent on addition of the exonuclease.

Item Type: Paper
Additional Information: Hoss, M Robins, P Naven, T J Pappin, D J Sgouros, J Lindahl, T Research Support, Non-U.S. Gov't England The EMBO journal EMBO J. 1999 Jul 1;18(13):3868-75.
Uncontrolled Keywords: Amino Acid Sequence Animals Base Pair Mismatch/genetics Catalysis Cell Nucleus/enzymology Chromosomes, Human, Pair 3/genetics Cloning, Molecular DNA Ligases/metabolism DNA Polymerase II/chemistry/genetics DNA Polymerase III DNA Polymerase beta/metabolism DNA Repair/genetics DNA, Single-Stranded/genetics/metabolism Escherichia coli/enzymology/ genetics Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/chemistry/ genetics/isolation & purification/ metabolism Humans Mass Spectrometry Molecular Sequence Data Molecular Weight RNA, Messenger/genetics/metabolism Rabbits Recombinant Fusion Proteins/biosynthesis/isolation & purification/metabolism Sequence Homology, Amino Acid
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > DNA polymerase
bioinformatics > genomics and proteomics > analysis and processing > molecular interaction processing
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning

organism description > bacteria > escherichia coli
Investigative techniques and equipment > spectroscopy > mass spectrometry
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > polypeptides
CSHL Authors:
Communities: CSHL labs > Pappin lab
Depositing User: Kathleen Darby
Date: 1 July 1999
Date Deposited: 28 Apr 2014 13:19
Last Modified: 28 Apr 2014 13:19
PMCID: 1171463
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29827

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