Ibotenate lesions of the hippocampus impair spatial learning but not contextual fear conditioning in mice

Cho, Y. H., Friedman, E., Silva, A. J. (January 1999) Ibotenate lesions of the hippocampus impair spatial learning but not contextual fear conditioning in mice. Behavioural Brain Research, 98 (1). pp. 77-87. ISSN 0166-4328 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10210524
DOI: 10.1016/S0166-4328(98)00054-0

Abstract

Recently, gene targeting and other mouse transgenic techniques have been used to study the cellular mechanisms underlying learning and memory mechanisms in the hippocampus. A key assumption of many of these studies is that lesions of the hippocampus have a similar impact on learning and memory in mice and in rats. Here, we used axon-sparing ibotenate lesions to determine whether damage to the hippocampus disrupts spatial learning and contextual conditioning in mice, as it is known to do in rats. Our results demonstrated that hippocampal lesions impair performance in the hidden-platform version of the water maze under a variety of experimental conditions. Neither keeping the start site constant, nor prior training with the visible-platform task fully rescued the spatial learning deficits of the lesioned mice. As previously shown in rats, the lesions left the performance of the mice intact in the visible-platform version of the water maze, indicating that they do not affect all types of learning, and that disruptions of sensory processing or motivation probably did not account for their deficits in the hidden-platform task. In contrast, the very same lesions did not affect either cued or contextual fear conditioning. These results confirm the involvement of the hippocampus in spatial learning in mice, and they also demonstrate that hippocampal-lesioned mice can show contextual fear conditioning. Thus, the behavioral findings presented here are crucial for the interpretation of transgenic experiments with the widely used water maze and fear-conditioning paradigms.

Item Type: Paper
Uncontrolled Keywords: Animals Association Learning/physiology Brain Mapping Conditioning, Classical/ physiology Escape Reaction/physiology Excitatory Amino Acid Antagonists Fear/ physiology Hippocampus/ physiology Ibotenic Acid Male Maze Learning/physiology Mental Recall/ physiology Mice Mice, Transgenic Orientation/ physiology Rats Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Space Perception/ physiology Species Specificity
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
Investigative techniques and equipment
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organism description > animal behavior > learning
CSHL Authors:
Communities: CSHL labs > Mainen lab
Depositing User: Kathleen Darby
Date: January 1999
Date Deposited: 28 Apr 2014 15:55
Last Modified: 30 Apr 2014 17:50
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29813

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