Selective acquisition of AMPA receptors over postnatal development suggests a molecular basis for silent synapses

Petralia, R. S., Esteban, J. A., Wang, Y. X., Partridge, J. G., Zhao, H. M., Wenthold, R. J., Malinow, R. (1999) Selective acquisition of AMPA receptors over postnatal development suggests a molecular basis for silent synapses. Nature Neuroscience, 2 (1). pp. 31-6.

URL: http://www.ncbi.nlm.nih.gov/pubmed/10195177
DOI: 10.1038/4532

Abstract

Early in postnatal development, glutamatergic synapses transmit primarily through NMDA receptors. As development progresses, synapses acquire AMPA receptor function. The molecular basis of these physiological observations is not known. Here we examined single excitatory synapses with immunogold electron-microscopic analysis of AMPA and NMDA receptors along with electrophysiological measurements. Early in postnatal development, a significant fraction of excitatory synapses had NMDA receptors and lacked AMPA receptors. As development progressed, synapses acquired AMPA receptors with little change in NMDA receptor number. Thus, synapses with NMDA receptors but no AMPA receptors can account for the electrophysiologically observed 'silent synapse'.

Item Type: Paper
Additional Information: 1097-6256 (Print) Journal Article
Uncontrolled Keywords: Aging/*metabolism Animals Animals, Newborn/growth & development/*metabolism Electrophysiology Hippocampus/growth & development/*metabolism Immunohistochemistry Male Microscopy, Electron Rats Rats, Sprague-Dawley Receptors, AMPA/*metabolism Receptors, N-Methyl-D-Aspartate/metabolism Synapses/*physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > AMPA receptor
Investigative techniques and equipment > electrophysiology
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
Investigative techniques and equipment > microscopy
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Communities: CSHL labs > Malinow lab
Depositing User: Kathleen Darby
Date Deposited: 30 Apr 2014 14:34
Last Modified: 30 Apr 2014 14:34
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29806

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