Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition

Soengas, M. S., Alarcon, R. M., Yoshida, H., Giaccia, A. J., Hakem, R., Mak, T. W., Lowe, S. W. (April 1999) Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition. Science, 284 (5411). pp. 156-9. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10102818
DOI: 10.1126/science.284.5411.156

Abstract

The ability of p53 to promote apoptosis in response to mitogenic oncogenes appears to be critical for its tumor suppressor function. Caspase-9 and its cofactor Apaf-1 were found to be essential downstream components of p53 in Myc-induced apoptosis. Like p53 null cells, mouse embryo fibroblast cells deficient in Apaf-1 and caspase-9, and expressing c-Myc, were resistant to apoptotic stimuli that mimic conditions in developing tumors. Inactivation of Apaf-1 or caspase-9 substituted for p53 loss in promoting the oncogenic transformation of Myc-expressing cells. These results imply a role for Apaf-1 and caspase-9 in controlling tumor development.

Item Type: Paper
Uncontrolled Keywords: Animals Apoptosis Caspases/genetics/ physiology Cell Division Cell Transformation, Neoplastic Cells, Cultured Cytochrome c Group/metabolism Genes, myc Genes, p53 Genes, ras Mice Mice, Nude Mitochondria/metabolism Mutation Neoplasms, Experimental/genetics/metabolism/ pathology Proteins/genetics/ physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Tumor Suppressor Protein p53/metabolism
Subjects: diseases & disorders > neoplasms
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Protease > caspases
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p53
CSHL Authors:
Communities: CSHL labs > Lowe lab
Depositing User: Kathleen Darby
Date: 2 April 1999
Date Deposited: 30 Apr 2014 14:05
Last Modified: 30 Apr 2014 14:05
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29804

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