Rapid dendritic morphogenesis in CA1 hippocampal dendrites induced by synaptic activity

Maletic-Savatic, M., Malinow, R., Svoboda, K. (1999) Rapid dendritic morphogenesis in CA1 hippocampal dendrites induced by synaptic activity. Science, 283 (5409). pp. 1923-7. ISSN 0036-8075 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10082466
DOI: 10.1126/science.283.5409.1923

Abstract

Activity shapes the structure of neurons and their circuits. Two-photon imaging of CA1 neurons expressing enhanced green fluorescent protein in developing hippocampal slices from rat brains was used to characterize dendritic morphogenesis in response to synaptic activity. High-frequency focal synaptic stimulation induced a period (longer than 30 minutes) of enhanced growth of small filopodia-like protrusions (typically less than 5 micrometers long). Synaptically evoked growth was long-lasting and localized to dendritic regions close (less than 50 micrometers) to the stimulating electrode and was prevented by blockade of N-methyl-D-aspartate receptors. Thus, synaptic activation can produce rapid input-specific changes in dendritic structure. Such persistent structural changes could contribute to the development of neural circuitry.

Item Type: Paper
Uncontrolled Keywords: 2-Amino-5-phosphonovalerate/pharmacology Animals Culture Techniques Dendrites/drug effects/physiology/ ultrastructure Electric Stimulation Excitatory Amino Acid Antagonists/pharmacology Green Fluorescent Proteins Hippocampus/cytology Luminescent Proteins/genetics Microscopy/methods Morphogenesis/drug effects Pseudopodia/drug effects/physiology/ ultrastructure Pyramidal Cells/physiology/ ultrastructure/virology Rats Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors/ physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Sindbis Virus/genetics/physiology Synapses/ physiology/ultrastructure Synaptic Membranes/physiology Synaptic Transmission
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
Investigative techniques and equipment > microscopy
organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
CSHL labs > Malinow lab
CSHL labs > Svoboda lab
Depositing User: Kathleen Darby
Date Deposited: 30 Apr 2014 13:47
Last Modified: 30 Apr 2014 13:47
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29802

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