A YAC-based physical map of the mouse genome

Nusbaum, C., Slonim, D. K., Harris, K. L., Birren, B. W., Steen, R. G., Stein, L. D., Miller, J., Dietrich, W. F., Nahf, R., Wang, V., Merport, O., Castle, A. B., Husain, Z., Farino, G., Gray, D., Anderson, M. O., Devine, R., Horton, L. T., Ye, W., Wu, X., Kouyoumjian, V., Zemsteva, I. S., Wu, Y., Collymore, A. J., Courtney, D. F., Tam, J., Cadman, M., Haynes, A. R., Heuston, C., Marsland, T., Southwell, A., Trickett, P., Strivens, M. A., Ross, M. T., Makalowski, W., Xu, Y., Boguski, M. S., Carter, N. P., Denny, P., Brown, S. D., Hudson, T. J., Lander, E. S. (August 1999) A YAC-based physical map of the mouse genome. Nature Genetics, 22 (4). pp. 388-93.

URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...
DOI: 10.1038/11967

Abstract

A physical map of the mouse genome is an essential tool for both positional cloning and genomic sequencing in this key model system for biomedical research. Indeed, the construction of a mouse physical map with markers spaced at an average interval of 300 kb is one of the stated goals of the Human Genome Project. Here we report the results of a project at the Whitehead Institute/MIT Center for Genome Research to construct such a physical map of the mouse. We built the map by screening sequenced-tagged sites (STSs) against a large-insert yeast artificial chromosome (YAC) library and then integrating the STS-content information with a dense genetic map. The integrated map shows the location of 9,787 loci, providing landmarks with an average spacing of approximately 300 kb and affording YAC coverage of approximately 92% of the mouse genome. We also report the results of a project at the MRC UK Mouse Genome Centre targeted at chromosome X. The project produced a YAC-based map containing 619 loci (with 121 loci in common with the Whitehead map and 498 additional loci), providing especially dense coverage of this sex chromosome. The YAC-based physical map directly facilitates positional cloning of mouse mutations by providing ready access to most of the genome. More generally, use of this map in addition to a newly constructed radiation hybrid (RH) map provides a comprehensive framework for mouse genomic studies.

Item Type: Paper
Additional Information: 1061-4036 (Print) Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.
Uncontrolled Keywords: Animals Chromosome Mapping *Chromosomes, Artificial, Yeast Contig Mapping Genetic Markers *Genome Mice/*genetics Models, Genetic *Physical Chromosome Mapping
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Stein lab
Depositing User: Kathleen Darby
Date: August 1999
Date Deposited: 30 Apr 2014 15:00
Last Modified: 30 Apr 2014 15:00
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29786

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