Introduction of green fluorescent protein into hippocampal neurons through viral infection

Malinow, R., Hayashi, Y., Maletic-Savatic, M., Zaman, S. H., Poncer, J-C, Shi, S. H., Esteban, J. A. (1999) Introduction of green fluorescent protein into hippocampal neurons through viral infection. In: Imaging neurons : a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. ISBN 0879695420

URL: http://books.google.com/books?id=eYg4ngEACAAJ&dq=i...

Abstract

In the past decade, advances in microscopy have been coupled with new methods of culturing and labeling cells to generate the new science of imaging. Imaging technologies allow investigators to look directly inside living cells and probe their form and function in unprecedented detail. This approach is revolutionizing many aspects of biomedical research, particularly neuroscience, in which visual techniques have traditionally been so important. This manual is the first comprehensive description of the range of imaging technologies being applied to living cells. With its origins in a laboratory course taught at Cold Spring Harbor Laboratory by the editors and contributors, it is packed with the kind of technical detail and practical advice that are essential for success, yet seldom found in the research literature. It covers both established methods and cutting-edge techniques such as multiphoton excitation microscopy and imaging of genetically engineered probes. Although it is neurons to which these technologies are most commonly applied, the methods described are readily adaptable to many other cell types. This book will therefore be an invaluable aid to investigators in cell and developmental biology and immunology as well as neuroscience who wish to take advantage of the extraordinary insights into cellular function offered by imaging technologies.

Item Type: Book Section
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein
organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
Investigative techniques and equipment > imaging
Investigative techniques and equipment > microscopy
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
CSHL labs > Malinow lab
Depositing User: Kathleen Darby
Date Deposited: 30 Apr 2014 16:44
Last Modified: 30 Apr 2014 16:44
URI: http://repository.cshl.edu/id/eprint/29785

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