Identification and characterization of a polyadenylated small RNA (s-poly A+ RNA) in dinoflagellates

Reddy, R., Henning, D., Liu, M. H., Spector, D., Busch, H. (1985) Identification and characterization of a polyadenylated small RNA (s-poly A+ RNA) in dinoflagellates. Biochemical and Biophysical Research Communications, 127 (2). pp. 552-7. ISSN 0006-291X (Print)0006-291x

URL: http://www.ncbi.nlm.nih.gov/pubmed/2579655
DOI: 10.1016/S0006-291X(85)80195-9

Abstract

A 104 nucleotide-long small RNA, referred to as s-poly A+ RNA, containing 30 adenosine residues on its 3' -end was found in dinoflagellates, purified and its nucleotide sequence was determined. The sequence is: (sequence text) The polyadenylation signal AAUAAA was not found in this RNA; this result indicates that the 30 nucleotide-long poly A on the 3' -end is either coded for by this gene, or the poly A chain is added on this small RNA by a mechanism different from that for polyadenylation of messenger RNAs. Two polyadenylated small RNAs identified previously were implicated in differentiation of chicken heart muscle cells (Deshpande, A. K., Jakowlew, S. B., Arnold, H., Crawford, P. A. and Siddiqui, M. A. Q. (1977) J. Biol. Chem. 252, 6521-6527), and in brain specific mRNA transcription (Sutcliffe, J. G., Milner, R. J., Gottesfeld, J. M. and Lerner, R. A. (1984) Nature 309, 237-241). This RNA is the first polyadenylated small RNA to be sequenced.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Chemical Phenomena Chemistry Dinoflagellida/*genetics Electrophoresis, Polyacrylamide Gel Poly A/*isolation & purification RNA/*isolation & purification RNA, Messenger RNA, Small Nuclear
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > dinoflagellate
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 26 Mar 2014 19:56
Last Modified: 23 Jan 2015 20:12
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29704

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