Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene

Efrat, S., Linde, S., Kofod, H., Spector, D., Delannoy, M., Grant, S., Hanahan, D., Baekkeskov, S. (1988) Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene. Proceedings of the National Academy of Sciences of the United States of America, 85 (23). pp. 9037-41. ISSN 0027-8424 (Print)0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/2848253

Abstract

Three pancreatic beta-cell lines have been established from insulinomas derived from transgenic mice carrying a hybrid insulin-promoted simian virus 40 tumor antigen gene. The beta tumor cell (beta TC) lines maintain the features of differentiated beta cells for about 50 passages in culture. The cells produce both proinsulin I and II and efficiently process each into mature insulin, in a manner comparable to normal beta cells in isolated islets. Electron microscopy reveals typical beta-cell type secretory granules, in which insulin is stored. Insulin secretion is inducible up to 30-fold by glucose, although with a lower threshold for maximal stimulation than that for normal beta cells. beta TC lines can be repeatedly derived from primary beta-cell tumors that heritably arise in the transgenic mice. Thus, targeted expression of an oncogene with a cell-specific regulatory element can be used both to immortalize a rare cell type and to provide a selection for the maintenance of its differentiated phenotype.

Item Type: Paper
Uncontrolled Keywords: Adenoma, Islet Cell/*genetics Animals Antigens, Viral, Tumor/genetics *Genes Insulin/biosynthesis/*genetics/secretion Insulinoma/*genetics Islets of Langerhans/*metabolism/secretion/ultrastructure Mice Mice, Transgenic *Oncogenes Pancreatic Neoplasms/*genetics Simian virus 40/genetics Tumor Cells, Cultured
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > insulin receptor
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 26 Mar 2014 20:25
Last Modified: 23 Jan 2015 20:12
PMCID: PMC282658
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29702

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