General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer

Sun, Q., Mayeda, A., Hampson, R. K., Krainer, A. R., Rottman, F. M. (December 1993) General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. Genes & Development, 7 (12b). pp. 2598-608. ISSN 0890-9369 (Print)0890-9369

[img]
Preview
PDF (Paper)
Krainer Genes and Development 1993.pdf - Published Version

Download (4Mb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/8276242
DOI: 10.1101/gad.7.12b.2598

Abstract

The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA. More importantly, SF2/ASF stimulates in vitro splicing of bGH intron D through specific interaction with the ESE sequences. However, another general splicing factor, SC35, does not bind the ESE sequences and has no effect on bGH intron D splicing. Thus, one possible function of SF2/ASF in alternative and, perhaps, constitutive pre-mRNA splicing is to recognize ESE sequences. The stimulation of bGH intron D splicing by SF2/ASF is counteracted by the addition of hnRNP A1. The relative levels of SF2/ASF and hnRNP A1 influence the efficiency of bGH intron D splicing in vitro and may be the underlying mechanism of this alternative pre-mRNA processing event in vivo.

Item Type: Paper
Uncontrolled Keywords: Alternative Splicing/*physiology Animals Cattle Enhancer Elements, Genetic/*physiology Growth Hormone/*genetics HeLa Cells Humans Nuclear Proteins/metabolism/*physiology RNA, Heterogeneous Nuclear/metabolism RNA-Binding Proteins/metabolism/*physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > exons > exon splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: December 1993
Date Deposited: 11 Mar 2014 19:36
Last Modified: 11 Mar 2014 19:36
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29603

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving