Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors

Caceres, J. F., Stamm, S., Helfman, D. M., Krainer, A. R. (September 1994) Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors. Science, 265 (5179). pp. 1706-9. ISSN 0036-8075 (Print)0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/8085156

Abstract

The opposing effects of SF2/ASF and heterogeneous nuclear ribonucleoprotein (hnRNP) A1 influence alternative splicing in vitro. SF2/ASF or hnRNP A1 complementary DNAs were transiently overexpressed in HeLa cells, and the effect on alternative splicing of several cotransfected reporter genes was measured. Increased expression of SF2/ASF activated proximal 5' splice sites, promoted inclusion of a neuron-specific exon, and prevented abnormal exon skipping. Increased expression of hnRNP A1 activated distal 5' splice sites. Therefore, variations in the intracellular levels of antagonistic splicing factors influence different modes of alternative splicing in vivo and may be a natural mechanism for tissue-specific or developmental regulation of gene expression.

Item Type: Paper
Uncontrolled Keywords: Adenovirus E1A Proteins/genetics *Alternative Splicing Base Sequence DNA, Complementary/genetics Exons Gene Expression Regulation Genes, Reporter HeLa Cells *Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Humans Molecular Sequence Data Nuclear Proteins/*genetics/*metabolism RNA-Binding Proteins Ribonucleoproteins/genetics/*metabolism Transfection Tropomyosin/genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Alternative Splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: 16 September 1994
Date Deposited: 11 Mar 2014 19:59
Last Modified: 11 Mar 2014 19:59
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29595

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