Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2

Mayeda, A., Krainer, A. R. (January 1992) Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2. Cell, 68 (2). pp. 365-75. ISSN 0092-8674 (Print)0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/1531115
DOI: 10.1016/0092-8674(92)90477-T

Abstract

When messenger RNA precursors (pre-mRNAs) containing alternative 5' splice sites are spliced in vitro, the relative concentrations of the heterogeneous ribonucleoprotein (hnRNP) A1 and the essential splicing factor SF2 precisely determine which 5' splice site is selected. In general, an excess of hnRNP A1 favors distal 5' splice sites, whereas an excess of SF2 results in utilization of proximal 5' splice sites. The regulation of these antagonistic activities may play an important role in the tissue-specific and developmental control of gene expression by alternative splicing.

Item Type: Paper
Uncontrolled Keywords: Adenovirus Early Proteins Base Sequence Cloning, Molecular Gene Expression Regulation/*genetics HeLa Cells *Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Humans Immunoblotting Molecular Sequence Data Nuclear Proteins/*metabolism Oncogene Proteins, Viral/genetics RNA Precursors/genetics/*metabolism RNA Splicing/genetics/*physiology RNA-Binding Proteins Ribonucleoproteins/*metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Alternative Splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear ribonucleoprotein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:
Communities: CSHL labs > Krainer lab
Depositing User: Matt Covey
Date: 24 January 1992
Date Deposited: 11 Mar 2014 20:48
Last Modified: 11 Mar 2014 20:48
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29590

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