A mutation in the effector region of Ras2 can be partially suppressed by alteration of a 'nonessential' region of Ras

Chen, L., Powers, S. (September 1994) A mutation in the effector region of Ras2 can be partially suppressed by alteration of a 'nonessential' region of Ras. Gene, 147 (1). pp. 107-10. ISSN 0378-1119 (Print)0378-1119

URL: http://www.ncbi.nlm.nih.gov/pubmed/8088533
DOI: 10.1016/0378-1119(94)90047-7

Abstract

Phenotypically normal revertants of budding yeast cells that contain the hyperactive RAS2Val19 allele often result from second-site mutations within the RAS2 locus itself. Several such intragenic revertants harboring a suppressed RAS2Val19 allele as their only RAS gene were analyzed. All such suppressors resulted from single amino acid substitutions that affected either: (i) the effector region of Ras2, (ii) the C-terminal CAAX box of Ras2, or (iii) residues known to be critical for GTP binding in Ras proteins. While these suppressor mutations completely suppressed the hyperactive phenotype induced by the Val19 substitution, they did not block the ability of Ras2 to promote growth at normal temperatures. These results suggest that in yeast, attenuation of Ras proteins can effectively block hyperactive phenotypes without completely blocking the growth-promoting function. A spontaneous intragenic mutation that restored function to an effector mutant was mapped to a 'nonessential' region of Ras proteins. Based on this genetic interaction with the effector region and the report that deletions of this region affect Ras/GAP interaction, we suggest that this region may have a functional role in Ras activation of target effectors.

Item Type: Paper
Uncontrolled Keywords: Base Sequence DNA Primers/chemistry Fungal Proteins/*chemistry/genetics GTP-Binding Proteins/*genetics Genes, Fungal Genes, Suppressor *Genes, ras Guanine Nucleotides/metabolism Molecular Sequence Data Mutation Protein Processing, Post-Translational Proto-Oncogene Proteins p21(ras)/*genetics Saccharomyces cerevisiae/*genetics *Saccharomyces cerevisiae Proteins Structure-Activity Relationship Valine/chemistry *ras Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > GTP binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Ras
CSHL Authors:
Communities: CSHL labs > Powers lab
Depositing User: Matt Covey
Date: 15 September 1994
Date Deposited: 25 Feb 2014 21:05
Last Modified: 25 Feb 2014 21:05
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29522

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