Analysis of the reduced growth factor dependency of simian virus 40-transformed 3T3 cells

Powers, S., Fisher, P. B., Pollack, R. (August 1984) Analysis of the reduced growth factor dependency of simian virus 40-transformed 3T3 cells. Molecular and Cellular Biology, 4 (8). pp. 1572-6. ISSN 0270-7306

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URL: http://www.ncbi.nlm.nih.gov/pubmed/6092919

Abstract

We have measured in a defined serum-free medium the platelet-derived growth factor (PDGF) and insulin requirements of normal Swiss 3T3 cells, simian virus 40-transformed 3T3 cells, and partial revertants of simian virus 40-transformed 3T3 cells. Swiss 3T3 cells displayed strong requirements for both PDGF and insulin. Both of these requirements were significantly diminished in simian virus 40-transformed 3T3 cells. Analysis of the PDGF and insulin requirements of the revertants indicated that the loss of either of these two growth factor requirements was not necessarily linked to the other; rather, the growth factor requirements were specifically associated with other parameters of transformation. The reacquisition of a PDGF requirement cosegregated with reversion to density-dependent growth inhibition, whereas reacquisition of a normal insulin requirement cosegregated with reversion to a normal growth dependence on calf serum. Anchorage dependence was dissociable from both growth factor requirements. The relationship between the PDGF requirement and density-dependent growth inhibition was further analyzed in normal 3T3 cells by measuring the PDGF requirement at different cell densities. At high cell densities, the requirement for PDGF became significantly greater. We suggest that at least in part the ability of transformed cells to grow to high saturation densities results from their loss of a requirement for PDGF.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Division/drug effects *Cell Transformation, Viral Cells, Cultured Culture Media Fibroblasts/cytology Insulin/*pharmacology Mice Platelet-Derived Growth Factor/*pharmacology Simian virus 40/*genetics
Subjects: Investigative techniques and equipment > cell culture
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > cell line

organism description > virus
CSHL Authors:
Communities: CSHL labs > Powers lab
Depositing User: Matt Covey
Date: August 1984
Date Deposited: 25 Feb 2014 21:42
Last Modified: 25 Feb 2014 21:42
PMCID: PMC368949
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29512

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