Partial "repair" of defective NEF genes in a long-term nonprogressor with human immunodeficiency virus type 1 infection

Carl, S., Daniels, R., Iafrate, A. J., Easterbrook, P., Greenough, T. C., Skowronski, J., Kirchhoff, F. (January 2000) Partial "repair" of defective NEF genes in a long-term nonprogressor with human immunodeficiency virus type 1 infection. Journal of Infectious Diseases, 181 (1). pp. 132-140. ISSN 0022-1899

URL: http://www.ncbi.nlm.nih.gov/pubmed/10608759
DOI: 10.1086/315187

Abstract

A 36-bp deletion close to the 5' end of NEF that impaired Nef function was found in a long-term nonprogressor with human immunodeficiency virus type 1 (HIV-1) infection, Forms containing an adjacent duplication of 33 bp were also frequently observed. The duplication showed no homology to the deleted region but restored the overall length of the first variable loop of Nef. NEF alleles carrying the duplication were active in class I major histocompatibility complex (MHC-I) down-modulation and enhancement of virus infectivity, However, they showed little activity in CD4 down-regulation and were unable to stimulate viral replication in human peripheral blood mononuclear cells. Our study indicates that the enhancement of virion infectivity and the stimulation of HIV-1 replication in lymphocytes are distinct functions of Nef, Our Endings also illustrate the capacity for repair of attenuating deletions in HIV-1 infection and suggest that a selective pressure for Nef-mediated MHC-I down-modulation and/or enhancement of virion infectivity exists.

Item Type: Paper
Uncontrolled Keywords: CYTOTOXIC T-LYMPHOCYTES HIV-1 INFECTION DOWN-REGULATION SERINE KINASE SIV VACCINE PROTEIN CD4 AIDS ACTIVATION ENDOCYTOSIS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organism description > virus
CSHL Authors:
Communities: CSHL labs > Skowronski lab
Depositing User: Matt Covey
Date: January 2000
Date Deposited: 29 Jan 2014 17:50
Last Modified: 29 Jan 2014 17:50
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29427

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