A novel reverse-genetic approach (SIMF) identifies Mutator insertions in new Myb genes

Rabinowicz, P. D., Grotewold, E. (November 2000) A novel reverse-genetic approach (SIMF) identifies Mutator insertions in new Myb genes. Planta, 211 (6). pp. 887-893. ISSN 0032-0935

URL: http://www.ncbi.nlm.nih.gov/pubmed/11144275
DOI: 10.1007/s004250000402

Abstract

We have developed a new strategy designated SIMF (Systematic Insertional Mutagenesis of Families), to identify DNA insertions in many members of a gene family simultaneously. This method requires only a short amino acid sequence conserved in all members of the family to make a degenerate oligonucleotide, and a sequence from the end of the DNA insertion. The SIMF strategy was successfully applied to the large maize R2R3 Myb family of regulatory genes, and Mutator insertions in several novel Myb genes were identified. Application of this technique to identify insertions in other large gene families could significantly decrease the effort involved in screening at the same time for insertions in all members of groups of genes that share a limited sequence identity.

Item Type: Paper
Uncontrolled Keywords: DNA insertions (identification) Myb transposable element transcription factor Zea (DNA insertions) TRANSCRIPTION FACTOR ZEA-MAYS ARABIDOPSIS MAIZE EXPRESSION SEQUENCE HOMOLOG ANTIRRHINUM MUTATIONS PRODUCTS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > transposable elements
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: November 2000
Date Deposited: 29 Jan 2014 15:53
Last Modified: 29 Jan 2014 15:53
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29421

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