Functional analysis of the leader peptide of the yeast gene CPA1 and heterologous regulation by other fungal peptides

Delbecq, P., Calvo, O., Filipkowski, R. K., Pierard, A., Messenguy, F. (October 2000) Functional analysis of the leader peptide of the yeast gene CPA1 and heterologous regulation by other fungal peptides. Current Genetics, 38 (3). pp. 105-112. ISSN 0172-8083

URL: http://www.ncbi.nlm.nih.gov/pubmed/11057443
DOI: 10.1007/s002940000140

Abstract

The 25-amino-acid leader peptide present at the 5' end of yeast CPA1 mRNA is responsible for the translational repression of that gene by arginine. We show here that the active domain of the yeast peptide is highly specific and extends over amino acids 6-23. The region between amino acids 6-21 is well conserved between similar peptides present upstream of CPA1-homologous genes in other fungi. The Neurospora crassa arg-2 peptide represses the expression of CPA1, whereas the peptide from Aspergillus nidulans has only a weak regulatory effect. Such results suggest that the N- and C-terminal amino acids of the peptide may influence its regulatory activity. We also show that the transcription start sites of CPA1 are not modified when the cells are grown in the presence of arginine, nor in a strain carrying an inactive peptide.

Item Type: Paper
Uncontrolled Keywords: yeast CPA1 leader peptide analysis heterologous regulation NEUROSPORA-CRASSA PHOSPHATE SYNTHETASE SMALL SUBUNIT ARGININE DNA REPRESSION EXPRESSION PATHWAY
Subjects: bioinformatics > genomics and proteomics > design > protein network design > peptide design
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Yin lab
Depositing User: Matt Covey
Date: October 2000
Date Deposited: 30 Jan 2014 21:52
Last Modified: 30 Jan 2014 21:52
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29390

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