Dual roles of the 11S regulatory subcomplex in condensin functions

Kimura, K., Hirano, T. (2000) Dual roles of the 11S regulatory subcomplex in condensin functions. Proceedings of the National Academy of Sciences of the United States of America, 97 (22). pp. 11972-11977. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/11027308
DOI: 10.1073/pnas.220326097

Abstract

Condensin is a multisubunit protein complex that reconfigures DNA structure in an ATP-dependent manner in vitro and plays a central role in mitotic chromosome condensation in Xenopus egg cell-free extracts. The Xenopus 13S condensin complex (13SC) is composed of two subcomplexes: an 8S core subcomplex (8SC) consisting of two structural maintenance of chromosomes (SMC) subunits (XCAP-C and -E) and an 11S regulatory subcomplex (11SR) containing three non-SMC subunits (XCAP-D2, -G, and -H). We report here the biochemical and functional dissection of this chromosome condensation machinery. Although both 8SC and 13SC can bind to DNA in vitro and contain the SMC ATPase subunits,only 13SC is active as a DNA-stimulated ATPase and supports ATP-dependent supercoiling activity. In the cell-free extracts, 13SC is the active form that binds to chromosomes and induces their condensation. Neither 11SR nor 8SC alone is able to bind to chromatin. Our results suggest that the non-SMC subunits have dual roles in the regulation of condensin functions: one is to activate SMC ATPases and the other is to allow the holocomplex to associate with chromatin in a mitosis-specific manner.

Item Type: Paper
Uncontrolled Keywords: MITOTIC CHROMOSOME CONDENSATION UBIQUITOUS PROTEIN FAMILY 13S CONDENSIN HISTONE H3 IN-VITRO PHOSPHORYLATION SEGREGATION MITOSIS DNA INTERACTS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > condensin complex
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
CSHL Authors:
Communities: CSHL labs > Hirano lab
Depositing User: Matt Covey
Date Deposited: 31 Jan 2014 16:10
Last Modified: 31 Jan 2014 16:10
PMCID: PMC17279
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29381

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