A chromodomain protein, Swi6, performs imprinting functions in fission yeast during mitosis and meiosis

Nakayama, J., Klar, A. J. S., Grewal, S. I. S. (April 2000) A chromodomain protein, Swi6, performs imprinting functions in fission yeast during mitosis and meiosis. Cell, 101 (3). pp. 307-317. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/10847685
DOI: 10.1016/S0092-8674(00)80840-5

Abstract

Inheritance of stable states of gene expression is essential for cellular differentiation. In fission yeast, an epigenetic imprint marking the mating-type (mat2/3) region contributes to inheritance of the silenced state, but the nature of the imprint is not known. We show that a chromodomain-containing Swi6 protein is a dosage-critical component involved in imprinting the mat locus. Transient overexpression of Swi6 alters the epigenetic imprint at the mat2/3 region and heritably converts the expressed state to the silenced state. The establishment and maintenance of the imprint are tightly coupled to the recruitment and the persistence of Swi6 at the mat2/3 region during mitosis as well as meiosis. Remarkably, Swi6 remains bound to the mat2/3 interval throughout the cell cycle and itself seems to be a component of the imprint. Our analyses suggest that the unit of inheritance at the mat2/3 locus comprises the DNA plus the associated Swi6 protein complex.

Item Type: Paper
Uncontrolled Keywords: MATING-TYPE LOCI EPIGENETIC INHERITANCE SCHIZOSACCHAROMYCES-POMBE DNA METHYLATION CHROMOSOME SEGREGATION HETEROCHROMATIN CHROMATIN DROSOPHILA REPRESSION DOMAINS
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
organs, tissues, organelles, cell types and functions > organelles, types and functions > meiosis
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Grewal lab
Depositing User: Matt Covey
Date: April 2000
Date Deposited: 31 Jan 2014 16:32
Last Modified: 31 Jan 2014 16:32
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29362

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving