A basal transcription factor that activates or represses transcription

Willy, P. J., Kobayashi, R., Kadonaga, J. T. (November 2000) A basal transcription factor that activates or represses transcription. Science, 290 (5493). pp. 982-984. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/11062130
DOI: 10.1126/science.290.5493.982

Abstract

We have identified an activity that is required for transcription of downstream promoter element (DPE)-containing core promoters in vitro. The purified factor was found to be the Drosophila homolog of the transcriptional repressor known as NC2 or Dr1-Drap1. Purified recombinant dNC2 activates DPE-driven promoters and represses TATA-driven promoters. A mutant version of dNC2 can activate DPE promoters but is unable to repress TATA promoters. Thus, the activation and repression functions are distinct. These studies reveal that NC2 (Dr1-Drap1) is a bifunctional basal transcription factor that differentially regulates gene transcription through DPE or TATA box motifs.

Item Type: Paper
Uncontrolled Keywords: II GENE-TRANSCRIPTION CORE PROMOTER ELEMENT NEGATIVE COFACTOR IN-VIVO BINDING NC2 DROSOPHILA MECHANISM TFIIA DR1
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Kobayashi lab
Depositing User: Matt Covey
Date: November 2000
Date Deposited: 31 Jan 2014 16:21
Last Modified: 31 Jan 2014 16:21
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29355

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