Loss of HCF-1-chromatin association precedes temperature-induced growth arrest of tsBN67 cells

Wysocka, J., Reilly, P. T., Herr, W. (June 2001) Loss of HCF-1-chromatin association precedes temperature-induced growth arrest of tsBN67 cells. Molecular and Cellular Biology, 21 (11). pp. 3820-3829. ISSN 0270-7306

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URL: http://www.ncbi.nlm.nih.gov/pubmed/11340173
DOI: 10.1128/mcb.21.11.3820-3829.2001

Abstract

Human HCF-1 is a large, highly conserved, and abundant nuclear protein that plays an important but unknown role in cell proliferation. It also plays a role in activation of herpes simplex virus immediate-early gene transcription by the viral regulatory protein VP16. A single proline-to-serine substitution in the HCF-1 VP16 interaction domain causes a temperature-induced arrest of cell proliferation in hamster tsBN67 cells and prevents transcriptional activation by VP16, We show here that HCF-1 is naturally bound to chromatin in uninfected tells through its VP16 interaction domain. HCF-1 is chromatin bound in tsBN67 cells at permissive temperature but dissociates from chromatin before tsBN67 cells stop proliferating at the nonpermissive temperature, suggesting that loss of HCF-1 chromatin association is the primary cause of the temperature-induced tsBN67 cell proliferation arrest. We propose that the role of HCF-1 in cell proliferation is to regulate gene transcription by associating with a multiplicity of DNA-bound transcription factors through its VP16 interaction domain.

Item Type: Paper
Uncontrolled Keywords: HERPES-SIMPLEX VIRUS AMINO-TERMINAL DOMAIN ACTIVATION DOMAIN REPRESSOR SIN3 HCF VP16 PROTEIN COMPLEX FAMILY LUMAN
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date: June 2001
Date Deposited: 15 Jan 2014 20:36
Last Modified: 15 Jan 2014 20:36
PMCID: PMC87041
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29333

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