Transcriptional activation: risky business

Tansey, W. P. (May 2001) Transcriptional activation: risky business. Genes & Development, 15 (9). pp. 1045-1050. ISSN 0890-9369

[img]
Preview
PDF (Paper)
Tansey Genes and Development 2001.pdf - Published Version

Download (357Kb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/11331599
DOI: 10.1101/gad.89650

Abstract

Transcriptional regulation is all about getting RNA polymerase to the right place on the gene at the right time and making sure that it is competent to conduct transcription. Traditional views of this process place most of their emphasis on the events that precede initiation of transcription. We imagine a promoter-bound transcriptional activator (or collection of activators) recruiting components of the basal transcriptional machinery to the DNA, eventually leading to the recruitment of RNA polymerase II and the onset of gene transcription. Although these events play a crucial role in regulating gene expression, they are only half the story. Correct regulation of transcription requires that polymerase not only initiates when and where it should, but that it stops initiating when no longer appropriate. But how are the signals from transcriptional activators, telling RNA polymerase to fire, terminated! Is this process governed by chance, with activators simply falling off the promoter at a certain frequency? Or is there some more direct mechanism, whereby activators are aggressively limited from uncontrolled promoter activation? A new article by Chi et al. (2001) suggests the latter may be true, and provides a mechanism for how a component of the basal transcription machinery can mark the activators it has encountered, sentencing them to an early death or banishing them from the nucleus. The ability of the basal transcriptional apparatus to mark activators provides an efficient way to limit activator function and ensures that continuing transcription initiation at a promoter is coupled to the continuing synthesis and activation of transcriptional activators.

Item Type: Paper
Uncontrolled Keywords: RNA-POLYMERASE-II UBIQUITIN-MEDIATED PROTEOLYSIS PROTEIN-KINASE SACCHAROMYCES-CEREVISIAE IN-VIVO PROTEASOME PATHWAY LIGASE COMPLEX FACTOR GCN4 C-JUN DEGRADATION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Tansey lab
Depositing User: Matt Covey
Date: May 2001
Date Deposited: 15 Jan 2014 22:12
Last Modified: 15 Jan 2014 22:12
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29316

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving