Transient oxygen-glucose deprivation induces rapid morphological changes in rat hippocampal dendrites

Piccini, A., Malinow, R. (November 2001) Transient oxygen-glucose deprivation induces rapid morphological changes in rat hippocampal dendrites. Neuropharmacology, 41 (6). pp. 724-729. ISSN 0028-3908

URL: http://www.ncbi.nlm.nih.gov/pubmed/11640926
DOI: 10.1016/S0028-3908(01)00095-8

Abstract

Previous studies have revealed that pyramidal neurons in the CA1 region of the hippocampus are extremely susceptible to ischemia-induced cell damage and undergo selective degeneration 2-4 days after the insult. Little is known about early morphological changes in neurons occurring immediately after ischemic insult. Using two-photon laser scanning microscopy we monitored dendritic morphology of cells expressing enhanced green fluorescent protein in response to a transient hypoxic-ischemic episode in organotypic hippocampal slice preparations. This type of vital imaging provides direct evidence of dendritic rearrangements in rat CA1 pyramidal neurons occurring as soon as 20 min after oxygen-glucose deprivation. We propose that dendritic reorganization, resembling that occurring after tetanic stimulation, may be an early stage response to compensate the loss of synapses caused by ischemia-induced neuronal injury.

Item Type: Paper
Uncontrolled Keywords: organotypic hippocampal slices virus-mediated transfection particle-mediated transfection oxygen-glucose deprivation TPLSM imaging CA1 EXPRESSION
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic arbors

organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines

organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat
CSHL Authors:
Communities: CSHL labs > Malinow lab
Depositing User: Matt Covey
Date: November 2001
Date Deposited: 16 Jan 2014 17:34
Last Modified: 16 Jan 2014 17:34
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29285

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