Stabilization but not the transcriptional activity of herpes simplex virus VP16-induced complexes is evolutionarily conserved among HCF family members

Lee, S., Herr, W. (December 2001) Stabilization but not the transcriptional activity of herpes simplex virus VP16-induced complexes is evolutionarily conserved among HCF family members. Journal of Virology, 75 (24). pp. 12402-12411. ISSN 0022-538X

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URL: http://www.ncbi.nlm.nih.gov/pubmed/11711630
DOI: 10.1128/jvi.75.24.12402-12411.2001

Abstract

The human herpes simplex virus (HSV) protein VP16 induces formation of a transcriptional regulatory complex with two cellular factors-the POU homeodomain transcription factor Oct-1 and the cell proliferation factor HCF-1-to activate viral immediate-early-gene transcription. Although the cellular role of Oct-1 in transcription is relatively well understood, the cellular role of HCF-1 in cell proliferation is enigmatic. HCF-1 and the related protein HCF-2 form an HCF protein family in humans that is related to a Caenorhabditis elegans homolog called CeHCF. In this study, we show that all three proteins can promote VP16-induced-complex formation, indicating that VP16 targets a highly conserved function of HCF proteins. The resulting VP16-induced complexes, however, display different transcriptional activities. In contrast to HCF-1 and CeHCF, HCF-2 fails to support VP16 activation of transcription effectively. These results suggest that, along with HCF-1, HCF-2 could have a role, albeit probably a different role, in HSV infection. CeHCF can mimic HCF-1 for both association with viral and cellular proteins and transcriptional activation, suggesting that the function(s) of HCF-1 targeted by VP16 has been highly conserved throughout metazoan evolution.

Item Type: Paper
Uncontrolled Keywords: ACCESSORY FACTOR HCF VP16 PROTEIN OCT-1 LUMAN TRANSACTIVATOR ACTIVATION DOMAIN DNA POLYPEPTIDES
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > virus > herpes simplex virus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > immediate early genes
organism description > virus
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date: December 2001
Date Deposited: 17 Jan 2014 21:05
Last Modified: 17 Jan 2014 21:05
PMCID: PMC116136
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29262

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