Chromosome condensation by a human condensin complex in Xenopus egg extracts

Kimura, K., Cuvier, O., Hirano, T. (February 2001) Chromosome condensation by a human condensin complex in Xenopus egg extracts. Journal of Biological Chemistry, 276 (8). pp. 5417-5420. ISSN 0021-9258

URL: http://www.ncbi.nlm.nih.gov/pubmed/11136719
DOI: 10.1074/jbc.C000873200

Abstract

13S condensin is a five-subunit protein complex that plays a central role in mitotic chromosome condensation. The condensin complex was originally identified and purified from Xenopus egg extracts and shown to have an ATP-dependent positive supercoiling activity in vitro. We report here the characterization of a human condensin complex purified from HeLa cell nuclear extracts. The human 13S complex has exactly the same composition as its Xenopus counterpart, being composed of two structural maintenance of chromosomes (human chromosome-associated polypeptide (hCAP)-C and hCAP-E) subunits and three non-structural maintenance of chromosomes (hCAP-D2/CNAP1, hCAP-G, and hCAP-H/BRRN) subunits. Human condensin purified from asynchronous HeLa cell cultures fails to reconfigure DNA structure in vitro. When phosphorylated by purified cdc2-cyclin B, however, it gains the ability to introduce positive supercoils into DNA in the presence of ATP and topoisomerase I. Strikingly, human condensin can induce chromosome condensation when added back into a Xenopus egg extract that has been immunodepleted of endogenous condensin. Thus, the structure and function of the condensin complex are highly conserved between Xenopus and humans, underscoring its fundamental importance in mitotic chromosome dynamics in eukaryotic cells.

Item Type: Paper
Uncontrolled Keywords: 13S CONDENSIN PROTEIN COMPLEXES HEAT REPEATS IN-VITRO HOMOLOG DNA PHOSPHORYLATION IDENTIFICATION SEGREGATION SEPARATION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Hirano lab
Depositing User: Matt Covey
Date: February 2001
Date Deposited: 17 Jan 2014 20:52
Last Modified: 17 Jan 2014 20:52
Related URLs:
URI: http://repository.cshl.edu/id/eprint/29258

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